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Literature summary extracted from

  • Green, A.R.; Hayes, R.P.; Xun, L.; Kang, C.
    Structural understanding of the glutathione-dependent reduction mechanism of glutathionyl-hydroquinone reductases (2012), J. Biol. Chem., 287, 35838-35848.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.7 binary complex structure with glutathionyl-menadione. The structure reveals a large H-site that could accommodate various substituted hydroquinones and a hydrogen network of three Tyr residues that could provide the proton for reductive deglutathionylation Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.5.7 C63A catalytic residue, mutant displays negligible activity Escherichia coli
1.8.5.7 Y195F catalytic residue, mutant displays negligible activity Escherichia coli
1.8.5.7 Y253F catalytic residue, mutant displays negligible activity Escherichia coli
1.8.5.7 Y296F catalytic residue, mutant displays negligible activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.7 0.34
-
 2-(glutathione-S-yl)-hydroquinone wild-type, pH 7.5, temperature not specified in the publication Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.7 Escherichia coli P42620
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.7 0.16
-
 mutant Y253F, pH 7.5, temperature not specified in the publication Escherichia coli
1.8.5.7 0.19
-
 mutant Y195F, pH 7.5, temperature not specified in the publication Escherichia coli
1.8.5.7 0.4
-
 mutant Y296F, pH 7.5, temperature not specified in the publication Escherichia coli
1.8.5.7 8.65
-
 wild-type, pH 7.5, temperature not specified in the publication Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.7 glutathione + 2-(glutathione-S-yl)-hydroquinone
-
 Escherichia coli glutathione disulfide + hydroquinone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.5.7 glutathionyl-hydroquinone reductase
-
 Escherichia coli
1.8.5.7 yqjG
-
 Escherichia coli